吴笛
特聘研究员 硕士导师
E-mail：diwulab@163.com
教育背景：
2020.06—至今，成都大学，药学与生物工程学院，特聘研究员；
2017.07—2020.05，成都大学，药学与生物工程学院，特聘副研究员
2015.08—2016.09，加拿大多伦多大学，化学学院，访问学者
2012.09—2017.07，四川大学，化学工程学院，应用化学，工学博士（硕博连读）
2008.09—2012.07，四川大学，化学工程学院，制药工程，工学学士
主要研究方向及成果：
研究方向：食品化学，活性小分子与蛋白质作用机理，光调控蛋白质策略
主要从事有机小分子与生物大分子互作的机理学研究及光调控蛋白质构象功能的应用策略技术。曾师从光调控蛋白质方向的权威科学家Andrew Woolley，系统细化了该领域涉及的知识与方法，并将该方向关键技术引进回国，突破了该光控技术难以在生物体中延长有效波段的技术瓶颈，并同我省高校及研究院所化学生物学领域的同行共享研究方法与光调控工艺的技术关键。近年来，主持国家自然科学基金青年基金1项，四川省科技厅应用基础面上项目1项，其它省部及厅局级项目4项，参与国家自科基金及四川省重大科技成果转化分析测试平台建设项目3项，累计可支配经费90余万。公开发表国内外学术论文50余篇，其中第一作者/通讯作者发表SCI论文20余篇（中科院一区TOP论文7篇、二区论文8篇），申报中国发明专利2项。担任Food Chemistry、Carbohydrate Polymers 、Food Hydrocolloids、Toxicology in Vitro、Food Bioscience、Luminescence等多个SCI期刊审稿人。承担《无机化学》、《食品分析》、《食品加工新技术研究进展》等课程的教学工作，指导大学生创新训练计划项目4项（国家级1项，省部级3项），指导学科竞赛获国家级、省部级奖多项，获拔尖创新人才培养荣誉学业导师称号，参编专著1部。已培养硕士研究生4人。近五年参加国际及全国性学术会议近10次，作国际会议学术汇报2次。
主要在研项目：
1. 国家自科基金青年基金项目：基于红光驱动的偶氮苯衍生物分子开关光调控蛋溶菌酶构象的研究（21808020，30万，2019-2021），主持，在研
2. 四川省科技厅科技计划应用基础面上项目：基于偶氮类食品着色剂光调控α-螺旋多肽构象的可行性研究及其应用（2018JY0151，10万，2018-2019），主持
3. 北京市食品添加剂工程技术研究中心开放基金：基于偶氮色素光控多肽片段（2019年，4.5万，2019-2020），主持
4. 农业部杂粮加工重点实验室开放基金：藜麦皂苷与功能性蛋白质的相互作用机制研究及功效评价（2018CC16，2万，2019-2020），主持
5. 肉类加工四川省重点实验室开放基金：肉制品防腐剂叔丁基羟基茴香醚及其代谢产物与血清白蛋白的相互作用研究（19-R-01，2019-2020）
6. 校人才引进启动项目，2017—2020，30万，主持。
近期20篇第一/通讯作者期刊论文：
1. Wu D*, Duan R, Geng F, Hu X, Gan N, Li H*. Comparative analysis of the interaction of mono-, dis-, and tris-azo food dyes with egg white lysozyme: A combined spectroscopic and computational simulation approach, Food Chemistry[J], 2019,284:180-187.
2. Wu D, Mei S, Duan R, Geng F*, Wu W, Li X, Cheng L, Wang C. How black tea pigment theaflavin dyes chicken eggs: Binding affinity study of theaflavin with ovalbumin, Food Chemistry[J], 2020,303:125407.
3. Wu D*, Liu D, Zhang Y, Zhang Z, Li H. Unravelling the binding mechanism of benproperine with human serum albumin: A docking, fluorometric, and thermodynamic approach, European Journal of Medicinal Chemistry[J], 2018, 146:245-250.
4. Wu D*, Wang J, Liu D, Zhang Y, Hu X. Computational and spectroscopic analysis of interaction between food colorant citrus red 2 and human serum albumin. Scientific Reports[J], 2019. 9：1615.
5. Duan Ran1, Wu D1,*, Tang L, Hu X, Cheng L, Yang H, Li H, Geng F. Interactions of the cis and trans states of an azobenzene photoswitch with lysozyme induced by red and blue light, Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy[J], 2020,229:117965.
6. Wu D, Huang S*. Transmission success probability analysis of vehicle users with mobile relays under mobility models. SCIENCE CHINA Information Sciences, 2020,63:169304
7. Di Wu, Mingxin Dong, Catherine V. Collins, Amirhossein Babalhavaeji, G. Andrew Woolley*, A Red-Light Azobenzene Di-Maleimide Photoswitch: Pros and Cons. Advanced Optical Materials, 4(2016) 1402-1409.
8. Di Wu, Jin Yan, Jing Wang, Qing Wang, Hui Li*. Characterization of interaction between food colourant Allura Red AC and human serum albumin: multispectroscopic analyses and docking simulations. Food Chemistry, 170 (2015) 423-429.
9. Di Wu, Jin Yan, Peixiao Tang, Shanshan Li, Kailin Xu, Hui Li*. Binding properties and structure-affinity relationships of food antioxidant butylated hydroxyanisole and its metabolites with lysozyme. Food Chemistry, 188(2015), 370-376.
10. Di Wu, Yuanming Zhai, Jin Yan, Kailin Xu, Qing Wang, Yuanzhi Li, and Hui Li*, Binding mechanism of tauroursodeoxycholic acid to human serum albumin: Insights from NMR relaxation and docking simulations. RSC Advances, 5 (2015) 11036-11042.
11. Di Wu, Jin Yan, Pingchuan Sun, Kailin Xu, Shanshan Li, Hongqi Yang, Hui Li*, Comparative analysis of the interaction of capecitabine and gefitinib with human serum albumin using 19F nuclear magnetic resonance-based approach. Journal of Pharmaceutical and Biomedical Analysis, 129 (2016) 15-20.
12. Di Wu, Jiajia Yang, Yuanli Cai, Xia Lin, Jin Yan, Hui Li*, Simultaneous determination of two amino bisphosphonates drugs by micellar electrokinetic chromatography. Journal of Chromatographic Science, 53 (2015) 1794-1800.
13. Di Wu, Shanshan Li, Kailin Xu, Lili Zhang, Xiaoqing Wu, Hui Li*, X-ray powder diffraction data for loratadine (C22H23ClN2O2). Powder Diffraction, 29 (2014) 193-195.
14. Di Wu, Peixiao Tang, Shanshan Li, Haozhong Luo, Hui Li*, X-ray powder diffraction data for gemcitabine, C9H11F2N3O4. Powder Diffraction, 30 (2015) 76-78.
15. Di Wu, Jin Yan, Keke Bai, Jing Wang, Qing Wang, Shujun Yan, Hui Li*, Analysis of interaction between tabseronine and bovine serum albumin. Journal of Sichuan University-Engineering Science Edition (EI), 2013, 45(4):181-185.
16. Yang H, Zeng Q, He Z, Wu D*, Li H. Interaction of novel aurora kinase inhibitor MK-0457 with human serum albumin: Insights into the dynamic behavior, binding mechanism, conformation and esterase activity of human serum albumin[J]. Journal of Pharmaceutical and Biomedical Analysis. 2020,178:112962.
17. Yang H, Zeng Q, He Z, Wu D*, Li H. Determination of the DNA binding properties of a novel PARP inhibitor MK-4827 with calf-thymus DNA by molecular simulations and detailed spectroscopic investigations[J]. New Journal of Chemistry, 2019,43:6702
18. Wang W, Gan N, Sun Q, Wu D*, Gan R, Zhang M, Tang P, Li H. Study on the interaction of ertugliflozin with human serum albumin in vitro by multispectroscopic methods, molecular docking, and molecular dynamics simulation[J], Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 2019,219:83-90.
19. Wang W, Gan N, Sun Q, Wu D*, Zhao L, Suo Z, Tang P, Li H. Binding properties of sodium glucose co-transporter-2 inhibitor empagliflozin to human serum albumin: spectroscopic methods and computer simulations[J], Journal of Biomolecular Structure and Dynamics, DOI: 10.1080/07391102.2019.1652688
20. Suo Z, Xiong X, Sun Q, Zhao L, Tang P, Hou Q, Zhang Y, Wu D*, Li H. Investigation on the Interaction of Dabrafenib with Human Serum Albumin Using Combined Experiment and Molecular Dynamics Simulation: Exploring the Binding Mechanism, Esterase-like Activity, and Antioxidant Activity[J]. Molecular Pharmaceutics, 2018, 15(12):5637-5645.
参加国内外重要学术会议
1. 2016，第二届国际蛋白质工程会议，加拿大渥太华
Photo-control of multimerizing elements by azobenzene cross-linkers. 2nd Protein Engineering Canada Conference, 2016, Ottawa. (Conference)
2. 2016，第六十届生物物理年会，美国洛杉矶
Photocontrol of Protein Affinity Reagents by Red Light using Azobenzene Switches. 60th Annual Meeting of the Biophysical-Society, 2016, Los Angeles. (Conference)
